Mass spectrometry, Protein Chemistry Facility
Research Institute of Molecular Pathology (IMP)
Institute of molecular biotechnology of the Austrian Academy of Sciences (IMBA)
Gregor Mendel Institute of Molecular Plant Biology (GMI)

PD-Nodes SimpatiQCo Software Tools Publications



Spectrummerger Node and Spectrummerger Standalone
Download and Installation (Standalone version)

Download the standalone version here. A manual is included.

Download and Installation (Node)

Functionality

The SpectrumMerger Node can be used to consolidate .raw files where separate id- and quan-scans have been recorded for each precursor. This is done by detecting for each precursor the two scans belonging together and merging the reporter ion area from the quan-scan into the id-scan. The merging is done by copying a selected m/z-range from the id-scan overwriting a selected m/z-range in the quan-scan. As theSpectrumMerger node is directly integrated into the PD workflow, the merged spectra can directly be used for further processing, e.g. performing a database search or exporting the spectra.

Usage

Simply include the SpectrumMerger node into a workflow, preceded by a Spectrum Source (e.g. Spectrum Selector or another spectrum manipulation node) and followed by the search node, or another spectrum manipulation node.

Figure 1: Example workflow using the SpectrumMerger node